مقاله انگلیسی ارزیابی ترانس ژنومیک به عنوان یک ابزار برای کشف ژن در Populus euphratica Oliv.
15 شهریور 1397 | 11:54
عنوان فارسی مقاله: ارزیابی ترانس ژنومیک به عنوان یک ابزار برای کشف ژن در Populus euphratica Oliv.
عنوان انگلیسی مقاله: An assessment of transgenomics as a tool for gene discovery in Populus euphratica Oliv.
مجله/کنفرانس: Plant Molecular Biology
رشته های تحصیلی مرتبط: محیط زیست، کشاورزی
گرایش های تحصیلی مرتبط: کشت و مراقبت
کلمات کلیدی انگلیسی: Transgenomics; Populus euphratica; Salt tolerance; Functional genomics; Arabidopsis thaliana
نمایه: scopus - master journals - JCR - medline
ایمپکت فاکتور(IF): 3.543(2017)
تعداد صفحات مقاله انگلیسی: 11
فهرست انگلیسی مطالب
Materials and methods
-Construction of the P. euphratica BIBAC library
-Characterization of the P. euphratica BIBAC library
-Transformation of cosmid DNAs into Agrobacterium tumefaciens
-Plant transformation of large DNA inserts with the floral dip method
-Phenotypic screening of primary plant transformants
-Analysis of the 002A1F06 clone
-Properties of the P. euphratica BIBAC library
-Phenotypic variation of the primary transformants
-Cosegregation analysis of inserts
-Salt tolerance of the transgenic plants with clone 002A1F06
-Salt tolerance genes in the 002A1F06 insert
-Transgenomics provides a novel approach for the study of functional genomics in woody plants
-Populus euphratica transgenomics creates a platform for functional genomics
-Transgenomics of P. euphratica can be a valuable resource for efficient gene discovery
نمونه متن انگلیسی
Transgenomics, a member of the omics family of methodologies, is characterized as the introduction of DNA from one organism into another on a genome-wide scale followed by the identification of recipients with altered phenotypes. This strategy allows investigators to identify the gene(s) involved in these phenotypic changes. It is particularly promising for woody plants that have a long life cycle and for which molecular tools are limited. In this study, we constructed a large-insert binary bacterial artificial chromosome library of Populus euphratica, a stress-tolerant poplar species, which included 55,296 clones with average insert sizes of about 127 kb. To date, 1077 of the clones have been transformed into Arabidopsis thaliana via Agrobacterium by the floral dip method. Of these, 69 transgenic lines showed phenotypic changes represented by diverse aspects of plant form and development, 22 of which were reproducibly associated with the same phenotypic change. One of the clones conferring transgenic plants with increased salt tolerance, 002A1F06, was further analyzed and the 127,284 bp insert in this clone harbored eight genes that have been previously reported to be involved in stress resistance. This study demonstrates that transgenomics is useful in the study of functional genomics of woody plants and in the identification of novel gene(s) responsible for economically important traits. Thus, transgenomics can also be used for validation of quantitative trait loci mapped by molecular markers.