مقاله انگلیسی پروتئین ضد انجماد نوع III به شدت بر حالت ژن های مربوطه در نواحی نوک ساقه سیب زمینی منجمد تاثیر میگذارد

15 شهریور 1397 | 09:56

مقاله انگلیسی پروتئین ضد انجماد نوع III به شدت بر حالت ژن های مربوطه در نواحی نوک ساقه سیب زمینی منجمد تاثیر میگذارد
عنوان فارسی مقاله: پروتئین ضد انجماد نوع III به شدت بر حالت ژن های مربوطه در نواحی نوک ساقه سیب زمینی منجمد تاثیر میگذارد
عنوان انگلیسی مقاله: Anti-freezing-protein type III strongly influences the expression of relevant genes in cryopreserved potato shoot tips
مجله/کنفرانس: Plant Molecular Biology
رشته های تحصیلی مرتبط: کشاورزی
گرایش های تحصیلی مرتبط: علوم باغبانی
کلمات کلیدی انگلیسی: Cold stress; Cryopreservation efficiency; Gene expression; Loading solution; Plant vitrification solution 2 (PVS2)
نوع نگارش مقاله: article
نمایه: scopus - master journals - JCR - medline
DOI: doi.org/10.1007/s11103-018-0743-8
ناشر: Springer
نوع ارائه مقاله: ژورنالی
نوع مقاله: ISI
سال انتشار مقاله: 2018
ایمپکت فاکتور(IF): 3.543(2017)
شاخص H_index: 152
SJR: 1.737
شناسه ISSN: 15735028
فرمت مقاله انگلیسی: PDF
تعداد صفحات مقاله انگلیسی: 9
کد محصول: EN55
فهرست انگلیسی مطالب
Abstract

Introduction

Materials and methods
-Plant material
-RNA extraction and quantitative real‑time PCR (qRT‑PCR) analysis
-Role of anti‑freeze protein in cryopreservation of potato shoot tips
-RNA extraction and detection of low temperature‑related genes following cryopreservation
-Statistical analysis

Results
-Induction of CBF1 and DHN1 genes in potato under low temperatures
-Regulation of CBF1 and DHN1 genes in shoot tips cryopreserved with AFP III
-Regulation of PM H+‑ATPase and PRCWP genesin shoot tips cryopreserved with AFP III
-Improvement of cryopreservation efficiency by AFP III

Discussion

Conclusion

References
نمونه متن انگلیسی
Abstract

Despite the availability of many studies reporting the benefits of anti-freeze protein III (AFP III) as a cryoprotectant, the role of AFP III in this process has not been well demonstrated using molecular analysis. In addition, AFP III has not been exploited in the cryopreservation of potato thus far. Therefore, we studied the effects of AFP III on the cryopreservation of potato (Solanum tuberosum cv. Superior). We found that CBF1 and DHN1 genes are low temperature-inducible in potato leaves (S. tuberosum cv. Superior). Transcript levels of these genes expressed in shoot tips cryopreserved with AFP III were higher than those of the controls. However, the optimal AFP III concentration required for strong induction of the genes was dependent on the type of cryoprotection solution to which the AFP III was added: 500 ng/mL worked best for PVS2, while 1500 ng/mL was optimal for LS. Interestingly, the involvement of AFP III in the cryoprotection solutions improved cryopreservation efficiency as compared to the control, and expression levels of the detected genes were associated with cryopreservation efficiency. This finding suggests that AFP III increased cryopreservation efficiency by transcriptional regulation of these genes, which might protect plant cell membranes from cold stress during cryopreservation. Therefore, we expect that our findings will lead to the successful application of AFP III as a potent cryoprotectant in the cryopreservation of rare and commercially important plant germplasms.
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