مقاله انگلیسی طراحی پپتیدهای ضد میکروبی از پایگاه داده cuttlefsh

05 شهریور 1397 | 18:10

مقاله انگلیسی طراحی پپتیدهای ضد میکروبی از پایگاه داده cuttlefsh
عنوان فارسی مقاله: طراحی پپتیدهای ضد میکروبی از پایگاه داده cuttlefsh
عنوان انگلیسی مقاله: Design of antimicrobial peptides from a cuttlefsh database
مجله/کنفرانس: Amino Acids
رشته های تحصیلی مرتبط: زیست شناسی
گرایش های تحصیلی مرتبط: میکروبیولوژی، زیست فناوری میکروبی
کلمات کلیدی انگلیسی: Antimicrobial peptides; Design; Sepia officinalis; Transcriptome; Predictive tools
نوع نگارش مقاله: پژوهشی - research
نمایه: scopus - master journals - JCR
DOI: doi.org/10.1007/s00726-018-2633-4
ناشر: Springer
نوع ارائه مقاله: ژورنالی
نوع مقاله: ISI
سال انتشار مقاله: 2018
ایمپکت فاکتور(IF): 2.906(2017)
شاخص H_index: 98
SJR: 1.135
شناسه ISSN: 0939-4451
فرمت مقاله انگلیسی: PDF
تعداد صفحات مقاله انگلیسی: 10
کد محصول: EN15
فهرست انگلیسی مطالب
Abstract

Introduction

Materials and methods

Peptide design

Peptide synthesis

Antimicrobial assays

Hemolytic activity

Circular dichroism

Results and discussion

Notes

Supplementary material

References
نمونه متن انگلیسی
Abstract

No antimicrobial peptide has been identified in cephalopods to date. Annotation of transcriptomes or genomes using basic local alignment Search Tool failed to yield any from sequence identities. Therefore, we searched for antimicrobial sequences in the cuttlefish (Sepia officinalis) database by in silico analysis of a transcriptomic database. Using an original approach based on the analysis of cysteine-free antimicrobial peptides selected from our Antimicrobial Peptide Database (APD3), the online prediction tool of the Collection of Anti-Microbial Peptides (CAMPR3), and a homemade software program, we identified potential antibacterial sequences. Nine peptides less than 25 amino acids long were synthesized. The hydrophobic content of all nine of them ranged from 30 to 70%, and they could form alpha-helices. Three peptides possessed similarities with piscidins, one with BMAP-27, and five were totally new. Their antibacterial activity was evaluated on eight bacteria including the aquatic pathogens Vibrio alginolyticus, Aeromonas salmonicida, or human pathogens such as Salmonella typhimurium, Listeria monocytogenes, or Staphylococcus aureus. Despite the prediction of an antimicrobial potential for eight of the peptides, only two—GR21 and KT19—inhibited more than one bacterial strain with minimal inhibitory concentrations below 25 µM. Some sequences like VA20 and FK19 were hemolytic, while GR21 induced less than 10% of hemolysis on human blood cells at a concentration of 200 µM. GR21 was the only peptide derived from a precursor with a signal peptide, suggesting a real role in cuttlefish immune defense.
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