مقاله انگلیسی گیرنده های کبد X و پروتئین های انعقادی تنظیمی عنصری استرول ترشح پروژسترون را دگرگون میکنند

27 شهریور 1397 | 13:15

مقاله انگلیسی گیرنده های کبد X و پروتئین های انعقادی تنظیمی عنصری استرول ترشح پروژسترون را دگرگون میکنند
عنوان فارسی مقاله: گیرنده های کبد X و پروتئین های انعقادی تنظیمی عنصری استرول ترشح پروژسترون را دگرگون میکنند و توسط گنادوتروپین کوریونی انسان در سلول های گرانولوزا لووتیونی انسان مرتب میشوند
عنوان انگلیسی مقاله: The liver X receptors and sterol regulatory element binding proteins alter progesterone secretion and are regulated by human chorionic gonadotropin in human luteinized granulosa cells
مجله/کنفرانس: Molecular and Cellular Endocrinology
رشته های تحصیلی مرتبط: پزشکی
گرایش های تحصیلی مرتبط: بیماری های داخلی
کلمات کلیدی انگلیسی: Progesterone; Liver x receptor; Sterol regulatory element binding protein; Human chorionic gonadotropin; Protein kinase A; Steroidogenic acute regulatory protein
نوع نگارش مقاله: پژوهشی - research
نمایه: scopus - master journals - JCR - medline
DOI: doi.org/10.1016/j.mce.2018.01.011
ناشر: الزویر - Elsevier
نوع ارائه مقاله: ژورنالی
نوع مقاله: ISI
سال انتشار مقاله: 2018
ایمپکت فاکتور(IF): 3.563(2017)
شاخص H_index: 125
SJR: 1.629
شناسه ISSN: 0303-7207
فرمت مقاله انگلیسی: PDF
تعداد صفحات مقاله انگلیسی: 12
کد محصول: EN114
فهرست انگلیسی مطالب
Abstract

Introduction

Materials and methods
-Isolation of human granulosa cells
-Cell treatments
-Ovine mixed luteal cell isolation and treatment
-Progesterone analysis
-Cholesterol efflux assay
-LDL uptake assay
-Total intracellular cholesterol analysis
-Semi-quantitative real-time PCR (QPCR)
-Western blot
-Statistical analysis

Results
-Effect of LXR agonist and/or SREBP inhibitor on P4 secretion and cholesterol metabolism
-Effect of hCG, LXR agonist and/or SREBP inhibitor on expression of LXR and SREBP target genes
-Effects of PKA inhibitor on hCG-mediated changes in LXR target genes and LDLR
-Effects of chronic hCG exposure on expression of LXR target genes and LDLR
-LXR regulation of STAR transcription in sheep and humans

Discussion

References
نمونه متن انگلیسی
Abstract

There is increased expression of liver x receptor (LXR) target genes and reduced low density lipoprotein receptor (LDLR) during spontaneous luteolysis in primates. The LXRs are nuclear receptors that increase cholesterol efflux by inducing transcription of their target genes. Transcription of LDLR is regulated by sterol regulatory element binding proteins (SREBPs). Human chorionic gonadotropin (hCG) prevents luteolysis and stimulates progesterone synthesis via protein kinase A (PKA). Thus, our primary objectives are: 1) Determine the effects of LXR activation and SREBP inhibition on progesterone secretion and cholesterol metabolism, and 2) Determine whether hCG signaling via PKA regulates transcription of LXR and SREBP target genes in human luteinized granulosa cells. Basal and hCG-stimulated progesterone secretion was significantly decreased by the combined actions of the LXR agonist T0901317 and the SREBP inhibitor fatostatin, which was associated with reduced intracellular cholesterol storage. Expression of LXR target genes in the presence of T0901317 was significantly reduced by hCG, while hCG promoted transcriptional changes that favor LDL uptake. These effects of hCG were reversed by a specific PKA inhibitor. A third objective was to resolve a dilemma concerning LXR regulation of steroidogenic acute regulatory protein (STAR) expression in primate and non-primate steroidogenic cells. T0901317 induced STAR expression and progesterone synthesis in ovine, but not human cells, revealing a key difference between species in LXR regulation of luteal function. Collectively, these data support the hypothesis that LXR-induced cholesterol efflux and reduced LDL uptake via SREBP inhibition mediates luteolysis in primates, which is prevented by hCG.
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